Harmonization and standardization are important when disease is dened by clinical biochemistry results. Evidence based medicine is the conscious, explicit and judicious use of current best evidence in making decisions about the care of individual patients. There are very few studies proving standardisation and it's application in laboratory medicine. The objective of this study is to verify the trueness and portability of patient reports by standardization of glucose estimation using two different methods in two different analysers. Methods of estimation compared are hexokinase and GOD-POD. Instruments used are Abbott Architect c8000 and ERBA XL-640 respectively. After the analysis, the results obtained are comparable and methods are traceable and therefore can be used interchangeably for patient report evaluation. Through this experiment we conclude that standardization enables uniform interpretability, which is important in integrating health systems into modern clinical environment