No abstract

The carboxy terminal octapeptide of cholecystokinin by 100 nM CCK 8 . The complex appeared to be (CCK 8 ) is a hormone that binds high affinity receptors in a internalised since it could not be removed by acid wash. number of tissues including pancreas and pancreatic When administered intra-arterially, the complex was raptumours. As part of our studies to develop effective gene idly removed from the circulation with no evidence of tartherapy for the treatment of pancreatic cancers, we have geted delivery to the pancreas. However, following a sininvestigated various gene delivery systems that depend on gle intraperitoneal dose, the pancreas accumulated 5-CCK 8 receptor targeting. In this paper, we describe the 8% of the total administered complex by 24 h. These synthesis of a CCK 8 -DNA complex designed to deliver results suggest that peptide-dependent gene delivery to foreign DNA to cholecystokinin receptor-positive cells.CCK receptor positive cells in vivo is feasible but, when CCK 8 was ligated to avidin and then complexed to linadministered directly into the circulation, diffusional barearised biotinylated DNA (pSV-CAT). The uptake of 32 Priers across the endothelium may limit distribution to perlabelled CCK 8 -DNA complex by rat pancreatic acini was ipheral tissues. Intraperitoneal administration therefore linear with time over 4 h with 65-70% of uptake inhibited may be a useful alternative for targeting the pancreas.