The current study amid to investigate association of Interleukin-17A with uropathogenic Escherichia coli among patients with urinary tract infection in karbala province, Iraq. Bacterial infections are widespread in urinary tract infections with a global extention. Uropathogenic E.coli (UPeC) is the most common causes of these infections. Out of 110 patients were examined by urologists for urinary tract infection, 25 patients showed positive result for UPEC and other 25 showed positive result for other bacterial pathogens. UPEC were diagnosed depended on the cultural, microscopical, biochemical examinations and confirm the identification by using Vitek2 system. Polymerase chain reaction was used to detection of four genes (pap C, cnfA, fim H, and fyu A). Interleukin-17A concentration in urine was measured by using ELISA kit. out of 110 urine samples, 56 (44.90%) with significant bacteriuria, 44(40%) with non-significant bacteriuria and 10 (9.09 %) with negative culture. The presence of UPEC among significant bacteriuria was 25/56 (44.64 %). The distribution of pap C, cnfA, fim H, and fyu A genes among UPEC were 17(68%), 17(68%), 16(64%) and 15(60%) respectively. Through UTI patients, 50 gave positive (121.70) pg/ml results compared to 30 of control (13. 94) pg/ml. Among uropathogenic Escherichia coli patients, 25 gave positive (92.80) pg/ml results, while 25 of other bacterial pathogens gave positive (15.40) pg/ml results.