Prostate cancer (PCa) is a malignant tumor that usually caused by many epigenetic alteration that lead to uncontrolled proliferation, differentiation and invasion to nearby tissues. Different types of treatment for patients of PCa have been applied including surgery, radiation, hormone and chemo-therapy. This work presented an experimental study for using low-cost and ecologically friendly Egypt plants' extracts (garlic, cinnamon and liquorice) that have been dissolved in DEMSO for the treatment of PCa. To evaluate the potential effect of the selected extracts on, PC3 cells, MTT assay, qRT-PCR and ELISA techniques were studied. The results showed that the garlic extract exhibited a significant cytotoxic effect against PC3. It was significantly increased the production of relative LDH in the treated cells rather than the extracts of cinnamon and liquorice. Also, it was found that garlic components effects on proapoptotic signaling including P53, Caspase-3 and modulates the Raf-1 signaling pathway, and downregulating the RAF/MEK/ERK pathway in PC3 cells compared with the effects of cinnamon and liquorice extractions. ELISA results indicated that the mean concentrations of IL-1α and IL-6 highly increased after 24h. In conclusion, the garlic extract was found to have an ability to adjust the secretion of pro-inflammatory cytokines. Meanwhile, garlic based herbal can be used as effective and harmless anti-cancer agent for PCa treatment.