Cervical cancer is a common malignant tumor in females. Orthodenticle homolog 1 (OTX1) serves a key role in the occurrence and progression of tumors. The present study aimed to investigate the role and potential mechanism of OTX1 in cervical cancer. OTX1 expression was analyzed by western blotting, reverse transcription-quantitative PCR and immunohistochemistry. MTT assay was performed to assess cell viability. EdU and colony formation assay were used to measure cell proliferation. Wound healing and Transwell assays were performed to measure cell migration and invasion. Western blot assay was performed for the assessment of protein expression. Gene set enrichment analysis (GSEA) was performed to analyze signaling pathways regulated by OTX1. Co-Immunoprecipitation assay was performed to confirm the interaction between OTX1 and Wnt9b. In cervical cancer tissue and cells, OTX1 was significantly upregulated. OTX1 overexpression promoted proliferation, migration and invasion of cervical cancer cells. OTX1 silencing significantly decreased cell proliferation, migration and invasion of cervical cancer. GSEA showed that OTX1 activated the Wnt signaling pathway. OTX1 silencing inhibited the increased levels of adenomatous polyposis coli (APC), glycogen synthase kinase (GSK)-3β and axis inhibition protein (AXIN)2 and decreased levels of Wnt9b and β-catenin. OTX1 overexpression decreased the levels of APC, GSK-3β and AXIN2 and increased levels of Wnt9b and β-catenin. However, XAV939 (a Wnt signaling inhibitor) and β-catenin silencing partly eliminated the effect of OTX1 overexpression on cervical cancer cells. OTX1 promoted the progression of cervical cancer by activating the Wnt signaling pathway.

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BackgroundCervical cancers are ranked the second-most hazardous ailments among women worldwide. In the past two decades, microarray technologies have been applied to study genes involved in malignancy progress. However, in most of the published microarray studies, only a few genes were reported leaving rather a large amount of data unused. Also, RNA-Seq data has become more standard for transcriptome analysis and is widely applied in cancer studies. There is a growing demand for a tool to help the experimental researchers who are keen to explore cervical cancer gene therapy, but lack computer expertise to access and analyze the high throughput gene expression data.DescriptionThe dbCerEx database is designed to retrieve and process gene expression data from cervical cancer samples. It includes the genome wide expression profiles of cervical cancer samples, as well as a web utility to cluster genes with similar expression patterns. This feature will help researchers conduct further research to uncover novel gene functions.ConclusionThe dbCerEx database is freely available for non-commercial use at http://128.135.207.10/dbCerEx/, and will be updated and integrated with more features as needed.

To terminate early stage pregnancy by combination of Mifepristone and Misoprostol is superior in high success rate, convenient use and less misery, but with the main badness of inducing irregular vaginal bleeding harmful to psychosomatic condition of puerpera. From March 1993 to December 1995, Yaoliuan Capsule (YLAC) was used by the authors to treat the drug-induced post-abortion vaginal bleeding and good effect was achieved. The study was introduced as follows. METHODS Clinical MaterialsThree hundred and twenty-three women, confirmed to be pregnancy of gestation age 49 days and without contraindication, were divided randomly into two groups, the tested group and the control group. Conditions of the 168 cases in the tested group were age: 18 -38 years, 28.5 years in average; menolipsis time : 33 -49 days, 41.2 days in average; last menstrual period: 3 -8 days, 5.8 days in average ; pre-pregnancy menstrual cycle: 21 -37 days, 27.8 days in average; gestation times: 1-4 times, 1. 8 times in average; labour times: 0-3 times, 1.3 times in average. Conditions of the 155 cases in the control group were age: 19 -39 years, 28.1 years in average; menolipsis time: 32 -49 days, 42.1 days in average; last menstrual period: 3 -8 days, 5. 9 days in average; pre-pregnancy menstrual cycle: 23 -38 days, 28.2 days in average; gestation times: 1 -4 times, 1.7 times in average; labour times: 0 -4 times, 1.4 times in average. Difference in conditions of the two groups was insignificant and therefore, they were comparable statistically.

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