No abstract

No abstract

In the present study, six known compounds were investigated that were isolated from the aerial parts of plant Dianthus helenae Vved. The structures of these compounds were identified as polypodine B (1), 2,3,20,22-diacetonide-20-hydroxyecdysone (2), 20-hydroxyecdysone (3), cyasterone (4), αecdysone (5) and 2-deoxy-α-ecdysone (6). Their structures were confirmed by NMR-, ESI-MS, and IR-spectroscopy. The compounds ( 5) and ( 6) are reported for the first time from this species. Furthermore, compounds (2) and ( 4) were isolated for the first time from the Caryophyllaceae family. In addition, all these phytoecdysteroids were investigated for a nootropic activity. Thus, the total phytoecdysteroids-containing preparation at a dose of 25 mg/kg increases two times the motor activity, an approximate reaction -four times and exploratory behavior -1.4 times compared to control animals.

An analysis method was developed for the separation and quantification of hesperidin, neohesperidin, neohesperidin dihydrochalcone and hesperetin by using HPLC-UV. Single factor experiments and Box-Behnken Designs were used to optimize separation of four flavonoids, in which a gradient elution method was adopted with 99% acetonitrile and 0.1% formic acid as mobile phases at a flow rate of 0.9 mL/min. A C18 column was used with a column temperature of 35℃. LODs and LOQs were below 0.84 µg/mL and 2.84 µg/mL, respectively. Linearity with good correlation coefficients (r>0.99, n=5) was attained, recovery rate of four flavonoids ranged from 88% to 130%, the RSD indicating results precision for analyzing hesperidin, neohesperidin, neohesperidin dihydrochalcone and hesperetin ranged from 1.2% to 4.6%. Finally, the 2 present method could be successfully applied to identify and quantify hesperidin, neohesperidin and hesperetin in Fructus Aurantii Immaturus and Pericarpium Citri Reticulatae.