Objectives: The objectives of this research was to measure the relative expression levels of vascular endothelial growth Factor-A (VEGF-A) in the deep dermal burns treated with human adipose-derived stem cell (hADSC) in collagen gel in each observational day (days 7, 14, 21, and 28).Methods: This study used 20 male Sprague Dawley rats, divided into four groups of observation days. Each rat received three burn injuries and then given different treatments (hADSC in collagen gel, collagen gel, and control). Deep dermal burn injury on the dorsal was made by placing a metal plate with 250°C for 15 s. Relative expression level of VEGF-A measurement with quantitative reverse transcription polymerase chain reaction.Results: On the 7th day, the relative expression level of VEGF-A in the wound treated with hADSC in collagen gel was significantly different from the scaffold collagen and control group (p<0.05), whereas the control and scaffold collagen group was not significantly different. The relative expression level of VEFG-A in wound treated with hADSC in collagen gel, collagen gel only, and control was (mean±standard error of the mean) 17.93±4.37, 7.54±2.63, and 5.44±1.59, respectively. On the next observation days, the result showed that the relative expression level of VEGF-A was not significantly different between the three treatments. The relative expression level of VEGF-A has decreases from day 7 to 28 days. The decrease of VEGF-A relative expression level hADSC in collagen gel group was significantly different on the 7th day to the 21st and 28th days (p<0.05).Conclusion: The provision of hADSC in scaffold collagen increases the relative expression of VEGF-A early in the wound healing process compared to the without a hADSC group.

Angiogenesis in the burn wound healing process is important. The application stem cell therapy in burn wound problem is expected to increase formation of new blood vessel so that the distribution of oxygen and nutrients working well. The purpose of this study was to known effect of human Adipose Derived Stem Cell (hADSC) in collagen gel on the average number of blood vessels based on time dependent. This study used 25 male rats Sprague Dawley who had received three internal wounds with different treatments. The treatments used hADSC in collagen gel, collagen gel only and without treatment. The rats were divided into 5 groups based on the duration of observation day 0, 7, 14, 21 and 28. Rat without burn wound were used as control. Observation blood vessels number using hematoxylin-eosin staining and calculated of average number blood vessels from five filed in five mice. Statistical analysis used ANOVA one way with significance value p<0.05. The average number blood vessels increase significantly until day 21 and decreased on day 28, both in untreated burn wound, collagen gel and hADSC in collagen gel. The treatment with hADSC in collagen gel was not significantly different from the collagen gel group or without treatment at day 7, 14 and 21. Even though the average of blood vessels of treatment with hADSC in collagen gel was not significantly different but our research had showed that treatment with hADSC in collagen gel was higher number of blood vessels than another group. Decrease of blood vessel number at 28 days indicated start of near homeostasis state and treatment hADSC in collagen gel has significantly decreased than another group. Our research showed that angiogenesis was time dependent and hADSC in collagen gel have not a role in increase of number blood vessels but we have indicated that hADSC might be have a role in qualitative improvement of angiogenesis.

Human adipose derived stem cells (hADSC) is expected to be an efficient solution for healing deep-dermal burns that help in wound closure. This study used 20 male Sprague dawley rats. Each rat received three wounds with different treatments (control, hADSC in collagen gel and collagen gel). Wound closure was observed at day 7th, 14th, 21th and 28th (sacrificed day) macroscopically, wound areas were measured and collagen densities were evaluated. Reduction of wound area showed significant difference between hADSC in collagen gel group and control group (p 0.001) and between hADSC in collagen gel group and collagen gel group. The hADSC in collagen gel group had a higher collagen density compared to control and collagen gel group. Application of hADSC in bovine collagen gel in rat deep-dermal burn models provided faster and better wound healing compared with control and bovine collagen gel group.