The epidemic of dengue hemorrhagic fever (DBD) in Indonesia, which occurs every year, has become a problem for the Indonesian society. Aedes aegypti mosquito is a vector of this disease. During this time the larvae of Aedes aegypti only has one exterminator is abate. In the long time exposure of single type insecticide will cause resistance. Therefore, it is necessary to fin d new bioinsectiside which is expected to have effects such as abate. The aims of this study are to offer technology, dosage, formulation and toxicity of new bioinsecticide granules toxin from ectract of papaya (Carica papaya) seed and leaf modified against Aedes aegypti larvae. Method :preparation of the extract by percolation method with 70% ethanol for seeds, and with water-ethanol 70% for leaf. Bioassay with active ingredients with thin layer chromatography. Formulation for 300 g Granules are: active substances papaya seed and leaf modified: 90%, seed flour 5%, filler: 5%. This was an experiment using a completely randomized design with 6 treatment groups. Each group contained 20 third instar larvae of Aedes aegypti. Concentrations of granules are 0, 30, 60, 90. 120, 150 ppm, with Temephos 1 % as a positive control. Data was analyzed using Probit analysis. Results: The result of phytochemical analysis contains secondary metabolites compounds of saponin, flavonoid and triterpenoid. Toxicity (LC 50 ) in this study is reported 107 ppm; 48 hours and LC 90 : 150 ppm, 48 hours. Conclusion: New Bioinsecticide Granules Toxin from extract of Papaya (Carica papaya) Seed and leaf Modified Against Aedes aegypti larvae possess technology, dosage, formulation, and larvicidal remarkable. Further investigations are needed to elucidate active ingredient responsible for larvacidal activity should be identified and utilized in preparing a commercial product.

Biotechnology material will be more meaningful if done with practice. STEM education in improving student skills and is very suitable when collaborated with biotechnology materials. This study aims to analyze the metacognitive skills and creative thinking of students at STEM education in senior high school. The analysis in this paper uses descriptive analysis using mix method analysis. The sample in this study was 150 senior high school students. Data was collected using questionnaires and observations. Data collection techniques of creative thinking using observation with five assessment instrument that is inquisitive, imaginative, persistent, collaborative, and disciplined and metacognitive skills use the MAI (Metacognitive Awareness Inventory) questionnaire test. The result showed the metacognitive skills with STEM education increases in good categories with an average of 73,5. The highest students’ creative thinking skills are persistent at 63,33% and the lowest was inquisitive 56,58%.

Colletotrichum  gloeosporioides is  a  disease  which  can  cause  significant yield  loss  of  cocoa.  The  objective  of  this  research  is  to  investigate  the  abilityof  antagonist  microbes,  Trichoderma  harzianum,  Trichoderma  koningii,  Bacillus subtilis  and Pseudomonas  fluorescens  in  controlling  gloeosporioides  biologically  in  laboratorium  condition.  The  experiment  was  carried  out  in  Crop  Protection  Laboratory,  Indonesian  Coffee  and  Cocoa  Research  Institute.  Results of  this  research  showed  that  antagonist  fungi,  T.  harzianum,  T.  koningii,  had  a stronger  ability  in  inhibiting  growth  of  C.  gloeosporioides about  83%  compared  to  the  ability  of  antagonist  bacteria,  B.  subtilis  and P.  fluorescens,  only about  49%. Key words: Growth  inhibition,  Colletotrichum  gloeosporioides,  Trichoderma  harzianum, Trichoderma koningii,  Bacillus subtilis, Pseudomonas fluorescens.

The poly methyl methacrylate (PMMA) research has been excecuted.. Methyl methacrylate is polymerized by the free radical inisiation with the suspension condition using benzoyl peroxyde inisiator, water as a solution and the gelatine stabilizer. In this research is excecuted as a standard ratio (part/part : monomer : water : stabilizer = 100 : 350 : (0,1 D I) with the inisiator to monomer ratio variation (part/part) are : 0,1/50 ; 0,175/50 ; 0,25/50 , the temperature variation are : 30 °C, 45 °C , 60 °C and the time polymerization until 320 minutes. The result showed that the optimum monomer polymerized was at the inisiator to monomer ratio 0,25/50, the temperature at 45°C and at the time polymerization until 300 minutes with the monomer conversion was 86 %. The Infra Red Spectroscopy analyses showed that there are poly methyl methacrylate in the product. The Gas Liquid Chromatography analysis showed the evolution of the monomer (methyl methacrylate concentration.. ABSTRAKTelah dilakukan penelitian pembuatan poli metil metakrilat (PMMA) . Metil metakrilat dipolimerisasi secara radikal bebas da lam kondisi suspensi menggunakan inisiator benzoil peroksida, pelarut air dan penstabil gelatin. Dalam penelitian ini digunakan perbandingan standar monomer : air: penstabil = 100 : 350 : (0,013 1) dengan variasi perbandingan inisiator/monomer (bagibag.):0,l /50; 0,175/50; 0,25/50 ; variasi temperatur 30°C, 45°C, 60°C serta variasi waktu polimerisasi sampai dengan 320 meniL Hasil penelitian menunjukkan bahwa monomer terpolimerisasi optimum terjadi i pada perbandingan inisiator /monomer 0,25/50 dan temperatur 45°C serta waktu polimerisasi 300 menit dengan monomer terpolimerisasi 86 %. Spektrum Infra Merah menunjukkan adanya poli metil metakrilat (PMMA). Spektrum Gas-Liquid Chromatography menunjukkan adanya evolusi konsentrasi monomer (matil metakrilat) setiap saat.

The use of chemical insecticides in the long term and high frequency will suppress insects (mosquitoes) and vectors to be tolerant until resistant. Reports of resistance from various species of mosquitoes found in multiple countries even occur in Indonesia in Surabaya. This study aimed to analyze the effect of granules bioinsecticides on the mixture of srikaya seeds (A. squamosa) and betel leaf (P. beetle) on the histopathology of mice (M. musculus) using true-experimental (the post-test only group design). Sample taken by simple random sampling and grouped into each control group and the treatment group totaling 25 mice. Data were analyzed using simple linear regression test. The results showed an overall p-value >0.05, meaning that there was no bioinsecticide effect of a mixture of betel leaf granules (Piper beetle) and srikaya seeds (Annona squamosa) on the histopathology of the kidney of mice (Mus musculus). Required safety testing of target animals in addition to mice and a preliminary examination is needed before the research is conducted so that the results are valid Keywords: bioinsecticide; histopatology; Mus musculus