The present paper describes a comparative study between sampling devices used In the official sampling plan for Arizona cottonseed and a device that provides a smaller sampling size. The official sample must be greater than 30 lbs from at least ten 3 lb probes/cottonseed lot, not exceeding 100 tons. This Is accomplished using a Probe-A-Vac pneumatic or corkscrew sampler with a 3 in. diameter X 50 In. probe. The alternative sampling device evaluated In this study uses a modified shop-vac wet-dry vacuum with a 0.75 in. diameter X 100 in. probe. Ten 1 lb probes were taken for a total sample size of 10 lbs. Aflatoxin concentrations in cottonseed samples taken using both sampling probes at adjacent points throughout the lot were determined with an AOAC thin-layer chromatographic method (26.052-26.059, 14th Ed., 980.20AH, 15th Ed.). Mean total aflatoxin concentrations for Probe-AVac vs shop-vac samples from 39 lots (1987-1988 crop year) and 21 lots (1988-1989 crop year) were 76.6 vs 68.5 and 39.5 vs 30.7 µg/kg, respectively. Long staple cottonseed was sampled using a long staple trier (LS Trier), an alternative for which may be the shop-vac system. Mean total aflatoxin concentrations for LS Trier vs shop-vac for 7 lots (1988-1989 crop year) were 9.4 vs 5.1, µg/kg respectively. Also, an ultracentrlfugal mill has proven to be highly versatile for both denuding and grinding cottonseed to be tested under both laboratory and nonlaboratory situations.
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