Bakteri selulolitik dan amilolitik mampu mengubah selulosa dan amilum menjadi glukosa serta mampu menghasilkan enzim selulase dan amilase. Bakteri selulolitik dan amilolitik diisolasi dari rumen sapi. Tujuan dari penelitian ini adalah untuk mendapatkan bakteri selulolitik dan amilolitik dari rumen sapi yang memiliki nilai indeks selulolitik dan amilolitik yang tinggi, serta mampu memenuhi syarat untuk dijadikan sebagai probiotik. Screening bakteri pada rumen sapi menghasilkan enam isolat yaitu AR, BR, CR, DR, ER, dan FR. Uji aktivitas selulolitik dilakukan pada substrat carboxy methyl cellulose (CMC) dan amilolitik pada amilum, uji ketahanan terhadap kondisi asam (pH 3), pengamatan pertumbuhan bakteri selama 30 jam, uji antagonistik terhadap bakteri patogen Aeromonas hydrophila, uji penempelan bakteri dan uji patogenisitas bakteri pada ikan. Hasil penelitian menunjukkan bahwa aktivitas selulolitik terbesar pada isolat AR (2,67 ± 0,35 cm) dan aktivitas amilolitik terbesar pada isolat AR (4 ± 0,60 cm). Hanya empat isolat (AR, BR, ER, dan FR) yang dilakukan uji lanjut. Keempat isolat mampu bertahan dalam kondisi asam pH 3 selama delapan jam dengan nilai OD terbesar pada isolat ER (1.137). Uji antagonistik menunjukkan bahwa isolat AR, ER, dan FR mampu menghambat pertumbuhan bakteri Aeromonas hydrophila. Isolat FR memiliki nilai antagonistik terbesar yang ditandai dengan terbentuknya zona hambat 17 mm. Uji penempelan bakteri untuk membuktikan bahwa isolat mampu menempel pada usus ikan. Uji patogenesitas pada isolat yang didapatkan guna membuktikan bahwa isolat tidak bersifat patogen terhadap inang. Berdasarkan hasil penelitian dapat disimpulkan bahwa isolat yang didapatkan dari rumen sapi termasuk bakteri selulolitik dan amilolitik yang telah memenuhi syarat sebagai kandidat probiotik pada ikan.Cellulolytic and amylolytic bacteria can transform cellulose and starch into glucose and produce the cellulase and amylase enzymes. These types of bacteria can be found in and isolated from cow’s rumen. Thus, the purpose of this study was to obtain potential cellulolytic and amylolytic bacteria from cow’s rumen with a high cellulolytic and amylolytic index value and can be qualified as probiotics. The screening of bacteria in the cow’s rumen produces six isolates i.e. AR, BR, CR, DR, ER, and FR. The parameters observed were: the cellulolytic activity in carboxymethyl cellulose (CMC) substrate, amylolytic activity on starch substrates, resistance test to acidic conditions (pH 3), bacterial growth for 30 hours, antagonistic to pathogenicity test to bacteria Aeromonas hydrophila, bacterial attachment test, and bacterial pathogenicity test in fish. The results showed that the largest cellulolytic and amylolytic activities were observed in AR isolates with a value of 2.67 ± 0.35 cm) and 4 ± 0.60 cm, respectively. Only four isolates (AR, BR, ER, and FR) were used in further tests. The four isolates were able to survive in the acidic conditions of pH 3 for 8 hours with the largest (OD) value was achieved by ER isolates (1,137). The growth of each isolate was different. The antagonistic test showed that the three isolates could inhibit the growth of A. hydrophila. FR isolates had the greatest antagonistic values characterized by the formation of an inhibition zone of 17 mm. Bacteria attachment test proved that the isolates were able to stick in the fish gut. The pathogenicity tests also proved that the isolates were not pathogenic to the host.
Kecamatan merupakan salah satu lembaga pemerintahan pelayanan masyarakat dan disebut dengan pelayanan publik. Kegiatan pelayanan masyarakat di Kecamatan Jangkar saat ini masih dilakukan secara konvensional sehingga membutuhkan waktu lama dan mengharuskan pemohon untuk mendatangi kantor terlebih dahulu. Tujuan dibuatnya Sistem informasi pelayanan masyarakat di Kecamatan Jangkar berbasis website dan via whatsapp gateway ini dapat memudahkan masyrakat dalam pembuatan surat secara online. Sehingga pemohon hanya melakukan registrasi dan mengisi persyaratan tanpa harus mendatangi kantor. Pemohon juga bisa melakukan pengajuan yang akan ditandatangani online oleh Camat. Pemohon juga bisa melihat status surat yang telah diajukan, dan ketika surat telah selesai pemohon akan mendapat notifikasi via whatsapp gateway. Sehingga hal tersebut bisa untuk meningkatkan kualitas pelayanan di Kantor Kecamatan Jangkar. Sistem ini juga menggunakan metode waterfall dan jenis penelitian kualitatif.
Fetal growth restriction is a prevalent pregnancy issue linked to numerous unfavorable postnatal outcomes. Suboptimal uterine-placental perfusion and inadequate fetal feeding are the vital pathophysiologic causes. Midwives play a crucial role in informing pregnant women about vitamin D inadequacy. This paper aims to determine the difference between midwives' prior and post-webinar knowledge levels. It was a cross-sectional study of individuals who participated in a webinar on November 21, 2021. Subjects were selected using a total population sampling approach from all midwives who participated in the webinar and completed pre-and post-tests. The difference between knowledge levels was evaluated using the difference between the pre-test and post-test scores in the general category. There was an increase in the number of participants who successfully answered the five-question component, a decrease in the number of participants who correctly answered the two-question section, and no change in the number of subjects who correctly answered question number one. The average score on the post-test was higher (67.28) than the pre-test (63.10), while the median score was the same at both time points. After attending a webinar, there was an increase in the proportion of subjects with a high level of knowledge (11.4% to 24%) and a drop in the proportion of subjects with a poor level of knowledge (32.3% to 27.1%). In conclusion, there was an improvement in the knowledge level of midwives before and after the webinar participation.
Government policies play an important role in world industry. Industrial policy is about wise government decisions in its efforts to change sentiment between industry and other economic sectors in a meaningful way, related to the sector's contribution to the economy, response rate, and income per capita. This research is part of a descriptive qualitative research on the diversity of industrialization in Indonesian government policies using various sources.
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