Dug wells are the most common and widespread well construction used to collect groundwater for the community, including residents in Karangmuncang Village, Cigandamekar District, Kuningan Regency. There are 1,552 residents who use dug wells as a means of clean water, there are 137 dug wells that have a high risk of contamination and the Escherichia Coli (E.coli) bacteria content of 2 MPN/100 ml was found. The purpose of this study is to determine whether there is a relationship between the physical condition of dug wells and the presence of Escherichia coli bacteria in Karangmuncang Village, Cigadamekar District, Kuningan Regency in 2022. This type of inferential research with cross sectional design, a sample of 68 dug wells. This research was conducted using a checklist sheet and the MPN laboratory test. The analysis was carried out using univariate and bivariate analysis in the form of Spearmen Rank analysis. The results of the univariate analysis showed that most of the physical conditions of dug wells had a high risk of pollution as many as 46 dug wells, most of the water did not meet the quality standards of Permenkes 32 of 2017 as many as 61 samples. Bivariate analysis of the two variables studied showed p = 0.086 (p>0.05) which means that there is no significant relationship between the physical condition of dug wells and the presence of Escherichia coli bacteria in Karangmuncang Village, Cigandamekar District, Kuningan Regency, r = 0.21 . and the direction of the correlation is positive. Based on the results of the Rank Spearmen test there is no significant relationship, the nature of the relationship is weak and the direction of the correlation is positive. It is ensured that the community boils clean water until it boils, the village government is encouraged to participate in monitoring and monitoring the quality of dug well water, the Cigandamekar health center conducts periodic checks of the water quality of the dug wells