Refractive error is one of the biggest eye problems in young people. The most prevalent Refractive error, myopia, is continuously increased. The myopia degree impact results in serious eye complication. Current studies report that myopia is associated with genetic and hormonal mechanism. Second to Fourth-Digit Ratio (2D:4D) on the other hand, has been reported as having a relationship with genetic and hormonal mechanism. The study was aimed to observe association between Second to Fourth-Digit Ratio (2D:4D) and myopia cases in medical students of Medical Faculty of Jenderal Soedirman University. This was a quantitative observational analytic research with cross sectional approach. One hundred students, divided into myopia and emmetropia group, was recruited for the study. Digit measurement was done using a computer-based method by two independent observers, whereas eye refraction test was conducted by an independent Refractionist. Independent t-test on 2D:4D ratio showed no difference in values (p>0,05) between myopia and emmetropia group. As conclusion, study reported that there was no significant association between second to fourth-digit ratio (2D:4D) with myopia cases in medical students of Medical Faculty of Jenderal Soedirman University.