the Biological Laboratory, Ottawa, for study and experimi^nts.Investigations were continued in the field and in the laboratories during the winter of and the following summer. The disease was found to exist in various parts of southern Alberta, and bj October 31, 1906, 412 animals had been destr'ived.At the Lethbridge quarantine station breeding experiments were conducted with the object of ascertaining whctlier app.nrently recovered mares would bear healthy offspring or would rem.iin sterile. I)i.«eased and healthy stallions were employed in these experiments. Several slightly affected mares were spayed in the hope that such cases could je spared destruction and usefully employed for work purposes only. The search for the dourine parasite Trypanosoma cquiperdum was kept up continuously both at Lethbridge and at Ottawa. Microscopical exami.ia'ions were made of great numbers of specimen.^; dogs and other laborator>' animaia were inoculated with blood and material obtained from diseased tissues, but all were negative in result and every attempt to detect the parasite mot with failure.At the Biological L.iboratory, Ott.^wa, Higgins and Watson had made a cytological study of the blood of dourined horses comparatively with the blood of normal horses and in certain diseases. It was found that the leneoeyto formula in dourine differed from that in normal horses and in the other diseases '' lied, and was of somo diagnostic value. A i)athoge:iio trypanosome, Tr. gamhiense, was maintained and studied in a series of laboratory animals, mainly with the idea of acquiring a knowledge of the habits ni.d eharaeteristics of a blood-haunting protozoan parasite elosel.v allied to that of dourine. In October, 1906, Watson took over the investigation of dourine and removed to Letiibridge. In November and 1 ' oember of that year he discovered trypanosomes in tlie blood of native "cotton-tail ' rabbits and in a species of northern deer-mouse caught on the quarantine grounds at Lethbridge. These findings created much interest at the time, being the first record of trypanosomes found in mammalian blood in Canada.
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