Blood samples were obtained from 78 randomly selected, healthy, unrelated individuals from both sexes and were examined in order to estimate the allele and genotype frequencies of D1S80 locus. DNA was extracted using Chelex 100 extraction method (1). The amplification was carried out according to the instructions of AmpliFLP D1S80 PCR amplification kit (2). The PCR products were analyzed using a vertical denaturating polyacrylamide gel electrophoresis. The gels were silver stained. The statistical analysis was done by the gene count method and it revealed no significant deviation from Hardy-Weinberg expectations. The complete data including the comparison with various Turkish and different populations (3) is available at http://istanbul.edu.tr/enstituler/ forensic/popgen-02.htm.