Chía (Salvia hispanica L.) seed contains more oil and protein than other grains, has a high content of omega-3 linolenic acid, essential for human nutrition. Paraguay is currently among the five largest chía producer countries in the world, with 80 000 ha planted in 2013. The experiments were carried out during the months of March and October of 2017, the field work was made in the district of Chore-San Pedro, Paraguay departament, and laboratory evaluations in the Seed Analysis and Quality Laboratory of Agrarian Sciences, National University of Asunción-Paraguay. The objective of this investigation was to determine the physiological quality effect on chia seeds with differents paraquat moments application. The variables study consisted in evaluate the seeds germinative power, calculate the vigor by measuring length of seedlings and accelerated aging, also estimate different harvest times yield. The randomized complete blocks design was used with 4 treatments with 4 repetitions. The obtained results were analyzed by ANOVA and the means were compared using the Tukey test at 5% probability. The Paraquat herbicide was applied as a desiccant to accelerate and standardize the chia harvest at different maturing times, called as early, middle and late. In all the analyzed variables it is shown that the early application obtained the best results concluding that the application of paraquat in early harvest favors the yield and the final quality of the seeds.