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An investigation of methods for the extraction and analysis of lipids from human arteries is described. The analytical procedure developed has been tested for completeness of separation and absence of artefact formation. It can be applied to lipid mixtures amounting to as little as 50 mg or in some cases even less. The lipids can be separated into phospholipids, free fatty acids, sterol esters, and glycerides plus free sterols, and the fatty acid mixtures from these fractions analysed by gas chromatography after methylation. Examples of such analyses are given.The partial examination of lipids extracted from body tissues and fluids is a common feature of many biochemical investigations, but only of recent years have attempts been made to analyse completely the mixtures of lipids so obtained. All recent investigations (e.8. 1-5) have begun with the separation of the total lipids into the major sub-groups (phospholipids, free fatty acids, free sterols, sterol esters and glyceryl esters) by chromatography on silicic acid, a technique first applied to this problem by Borgstr6rns. 7 and by Fillerup and Mead 8. The fatty acid composition of the separate

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