We aimed at evaluating the cardiovascular and metabolic responses to sub-maximal exercise tests in untrained non-athletic young adults and comparing them with those of trained and more active soccer players. Forty healthy young adult males (20 untrained non-athletic undergraduates and 20 trained soccer players) aged 20-35 years participated in the study. The participants performed the exercise tests using a mechanically braked magnetic ergometer bicycle. Blood pressure and heart rate (HR) of participants were measured at rest and during the exercise test. The steady-state oxygen uptake (VO2SS) of subjects was estimated from a standardised sub-maximal VO2 equation. Data indicated significantly (P<0.05) higher absolute VO2SS, relative VO2SS, work rate, exercise oxygen pulse and cardiac output, but lower steady-state HR and %HRmax among the soccer players compared with the untrained individuals. No significant differences were observed in exercise systolic blood pressure, rate pressure product, resting HR reserve and %HR reserve between the two groups. Pearson’s partial correlation test indicted independent relationships between VO2SS (relative and absolute) and oxygen pulse, steady-state HR, percentage of HRmax, percentage of HR reserve, resting HR and working HR reserve respectively. The present study indicated greater cardio-metabolic responses to sub-maximal exercise and higher aerobic fitness in trained soccer players compared with the untrained non-athletic individuals.