L-prolinamide, 5-oxo-l-prolyl-l-phenylanyl-4-hydroxy(5.19%). Antifungal activity of the ethyl acetate extract of the secondary metabolites were examined against Pythium spp MTCC 10247 and result revealed that the lag period of the fungi was double fold higher than the control and the compounds were fungi static because there was a 57% of growth reduction after 7 th day of incubation period. All the seven compounds were used for molecular docking study and result showed higher score value with L-prolinamide, 5-oxo-l-prolyl-lphenylanyl-4-hydroxy