In patients with a surgical profile, the humoral link of the immune system was studied for the production of 3 classes of immunoglobulins (IgA, IgM, IgG), the formation of circulating immune complexes (CIC), bactericidal activity of blood serum (BASK) in comparison with the clinical picture of the disease. Serum immunoglobulins were determined according to Mancini, CEC by the conventional method according to D. K. Novikov (1987), BASK by the method of V. I. Nikitenko and O. V. Bukharin.

With the creation of new measuring instruments equipped with automation elements and powerful computer systems, the prospect of a qualitatively new level of laboratory diagnostics has arisen. Currently, large diagnostic laboratories are being equipped with equipment from well-known companies in Japan, USA, Germany, Austria, Switzerland. Research opportunities in the field of hematology, clinical biochemistry, immunology, microbiology have expanded significantly.

The investigations of 13846 blood specimens for virus hepatitis В and C of somatic profile contingent of hospital and polyclinic departments are performed. The increase of the propagation rate of markers of virus hepatitis В (HBs antigen and anti-НВ cor) and antigen of virus hepatitis C among medical workers and patients of somatic profile in last years is revealed.

Serum immunoglobulins (A, G, M) are usually quantified by radial gel immunodiffusion (RID). It is based on measuring the diameter of the precipitation ring formed when the serum is added to the wells cut out in the agar layer in which the monospecific antiserum is preliminarily dispersed. The gel plates are placed in a humid chamber for 24-48 hours. At the end of the incubation, the diameter of the precipitation ring is measured. Under standard experimental conditions, the diameter of the precipitation ring is directly proportional to the concentration of the studied immunoglobulin. The amount of immunoglobulins is determined by constructing a calibration graph. The results of determining the concentration of serum immunoglobulins by the RID method are issued only on the 3rd day of the study. Clinicians are not always satisfied with the timing of the results and the quality of the study, since the latter depends on many factors - thoroughness (conducting the experiment, the quality and thickness of the gel, the scale of the schedule, etc.)

As many as 405 patients of therapeutic, surgical hospitals and ambulatory clinic patients are examined. Dysbacteriosis is revealed in 40,2% of the cases. The disorders of the intestine microbiocenosis are characterized by the tendency to allergization of the organism (high level of IgE).