SUMMARY The effect of low dose infusions of somatostatin on meal stimulated gastric acid secretion was studied in eight healthy volunteers by intragastric titration after a peptone test meal with radioimmunoassay control of the plasma concentrations of somatostatin and the pancreatic hormones glucagon and insulin. Infusion of somatostatin in a dose of 100 ng/kg/h, resulting in a plasma concentration of 13A4±2 1 pmol/l, inhibited acid secretion significantly, and in a dose of 800 ng/kg/h, with corresponding plasma concentration of 66-5±12-0 pmol/l the acid secretion was virtually abolished. Plasma concentrations of insulin and pancreatic glucagon decreased significantly during infusion of 200 ng/kg/h (24.5±7.5 pmol/l) and glucose concentrations increased. Serum gastrin was only significantly decreased during the highest dose of somatostatin. The range of plasma somatostatin concentrations obtained with the lower doses correspond to reported physiological variations. The results support the concept that somatostatin participates in the hormonal control of the pancreatic endocrine and the acid secretion.The particular localisation and shape of the somatostatin-producing D-cells in the gastric epithelium and the pancreatic islets suggests that somatostatin acts as a paracrine messenger. 1-4 Studies in dogs by Schusdziarra et at5 however, have indicated that somatostatin may act as a true hormone with a regulatory role in the homeostasis of ingested nutrients. Furthermore, studies in different experimental animals613 and human subjects1420 have shown that exogenous somatostatin is a potent inhibitor of gastric acid secretion and of the endocrine pancreas, and it has been suggested that somatostatin participates in the physiological control of these secretions as a hormonal mediator.5 20-21 The reported effects of somatostatin may be pharmacological, however, because plasma concentrations were generally not controlled.The present study deals with the effect of increasing doses of somatostatin on meal stimulated gastrin and gastric acid secretion under immunochemical control of the plasma concentrations of somatostatin. In addition we studied the effect on