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A monoclonal antibody against T-2 toxin was produced using a mouse assay). The antibody cross-reacted less to HT-2 toxin than T-2 antibodies immunized by subcutaneous injections into the shoulder with large previously described. Strong cross-reaction with the T-2 metabolites 3'OH immunogen doses. When this antibody was used in an indirect competitive T-2 and 3'OH HT-2 was noted. enzyme immunoassay, sensitivity to T-2 toxin was 10 ng/ml (0.5 ng per

A competitive enzyme-linked immunosorbent assay was used to screen for T-2 toxin in Fusarium sporotrichioides-infected corn. The assay detected T-2 toxin in diluted methanol extracts of corn samples at concentrations of 0.05 ng/ml. In infected corn samples, enzyme-linked immunosorbent assay and gas-liquid chromatography estimations of T-2 toxin concentrations were similar.

pruning wound of F. lateritium suspended in 5 gL sterile distilled water (SDW) was Field trials were conducted at four different dates in the winter and early spring of 1979-1980 and applied. The F. lateritium culture was also in 1981 in a commercial vineyard at Lawton, MI. In the 1979-1980 trials, a benomyl 50WP provided by M. V. Carter (Department of spray of 4.8 g/ L water (0.5 L per vine) gave significant control of ascospore infection by Eutypa Plant Pathology, Waite Agricultural armeniacae when pruning wounds on 2-yr-old wood were inoculated either on the day of pruning Research Institute, University of Adelaide, and spraying or 14 days later. A lower rate of benomyl 50WP, two rates of captafol 4F, and a Glen Osmond, South Australia). This biological control treatment of Fusarium lateritium gave little or no control. Pruning wounds inoculum was scraped off cultures declined in susceptibility over the 14-day period. No differences in susceptibility to infection were noted among the four dates of pruning. In the 1981 trial, benomyl 50WP showed significant disease growing on Difco potato-dextrose agar control at 1.2 and 9.6 g/ L water compared with water-treated control vines. (PDA) and suspended in SDW. Concentrations were determined using a hemacytometer. There were five vines per treatment in a completely randomized plot design. Eutypa dieback, also referred to ascospores by 65 and 100%, respectively After treatment, on the same day, 10 of previously as "dying arm" (12) or "dead (A. Trese, unpublished), the 20 pruning wounds per vine were each arm" (14), is an important disease of Macroconidial suspensions of Fusarium inoculated with 500 ascospores of F. grapevine worldwide, causing cankering lateritium Nees. were effective against E.