Real-time maps showing the intensity of the subskin blood flux reconstructed by the contrast variation in single-exposure (prolonged-exposure) speckle photography (left) and isolines of these maps (right)The dynamic bio-speckle patterns were generated by illumination of living tissue via laser light and were recorded using a standard digital CCD camera (768 x 494 pixels) at a frame rate of 25 frames/second. The exposure time varied from 10 Ps (for cross-correlation analysis of subsequent frames) to 1/60 s (for a single exposure mode). Speckle patterns were recorded as a distribution of gray values I(m,n) in digital form for each pixel (m,n) of the CCD matrix. In real-time operation the image analysis is performed during the time interval between subsequent (two or more) frames.
Abs ract : Joint development of a laser monitor for the real-time bio-tissue analysis is presented.The monitor is based on the digital dynamic laser speckle photography and deals with soft and hard bio-tissues. In soft tissues, the dynamic bio-speckles are formed in a scattered from a tissue laser light. An optically transparent model of hard bio-tissue was prepared and preliminary analysis of a stress field in the stressed model was performed using the dependence of the refractive index of transparent solids upon the state of stress and the double exposure speckle photography data. The refractive index of the stressed material was evaluated and the state of stress was reconstructed using the stress-optical law. t
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