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EChfhl2002coomassie brilliant blue R250 and after destaining different bands were observed.Results showed that frequency of two proteins (54328 and 38905 Dalton) in the acute form of infection was more than in the chronic form. These two bands may play an important role in candidosis pathogenesis, so further investigation is needed for characterization of these molecules.Cyptococcus neoformans has been subdivided into 3 varieties: Cr neofonnans var. grubii (serotype A), var. neoformans, (serotypes D), hybrid (serotype A D ) and var. gattii (serotypes B, C). 340 clinical, environmental and veterinary isolates from Argentina, Brazil, Chile, Colonibia, Mexico, Peru, Venezuela, Guatemala and Spain were divided by PCR-fingerprinting with a minisatellite specific primer (hf13) and RFLP analysis of the orotidine monophosphate pyrophosphorylase (CR4.5) and phospholipase (PLBZ) genes into 8 niolecular types.