Varicocele is an abnormal enlargement of the pampiniform venous plexus in the scrotum. The adverse effect of varicocele on spermatogenesis can be attributed to many factors such as an increased testicular temperature, increased intratesticular pressure, hypoxia due to attenuation of blood flow, reflux of toxic metabolites from the adrenal glands and hormonal profile abnormalities. FGL2 might play a protective role during sperm maturation in epididymis. FGL2 was found to be secreted from the principal cells into the tubule lumen where sFGL2 binds specifically to the nonviable, but not the viable, spermatozoa. This process forms sFGL2-protein complex that coats and envelops dying sperms to restrict release and spread of detrimental enzymes and immunogenic molecules from defective spermatozoa. The aim of study is to evaluate FGL2 in semen of patients with varicocele. We determined the presence of fgl2 in the seminal plasma via enzyme-linked immunosorbent assay [ELISA], whereas semen analysis was done as a routine investigation before separation of seminal plasma.fgl2 values in 55 infertile patients were significantly higher than in 25 healthy volunteers [p >0.001]. this result demonstrated strong association between infertility and level of fgl2 in the semen, fgl2 level are increased in infertility.