The present study was carried out at El-Mataana Agricultural Research Station, Agricultural Research center, Luxor Governorate (Latitude 25°41′N, Longitude 32°39′E) in three crop seasons 2018/19, 2019/20 and 2020/21 (PC, FR and SR) to evaluate stability and ratoon ability of five sugarcane genotypes, namely (G.84-47, G.2003-47, G.2004-27 and C.57-14 compared with the commercial variety GT.54-9) under three-row distances (80, 100 and 120 cm). A strip block design was used. The results recorded that, the new variety G.2004-27 had the high cane and sugar yields followed by the cultivated variety GT.54-9 without significant difference between them only in sugar yield trait, whereas the lowest values were recorded by C.57-14 genotypes. The genotype G.2004-27 and the commercial variety GT.54-9 got their highest cane and sugar yields under 100 cm row distance in SR crop. The commercial variety GT. 54-9 and G.2003-47 (new variety) had the 1 st rank of RA under 80 cm row distance for cane yield, while under 100 cm row distance the cultivar GT.54-9 ranked in 1 st where the new variety G.2003-47 ranked in 1 st one under 120 cm row distance in the same trait. Although, the promising variety G.2003-47 was the most stable genotype in cane and sugar yields across tested environments.