In a previous paper (Metzger and Podwiinska, 1967) evidence was presented that the development of agglutinability which occurs in ageing suspensions of T. pallidum (Hardy andNell, 1955, 1957; Metzger and Podwinska, 1965) (2) Each batch of treponeme suspensions under (i), (ii), and (iii), prepared anew for every immunization week, was tested for virulence by injecting 01 ml. of the suspension containing 29 million organisms at each of four sites on the shaven back of a rabbit, and 0 5 ml., 299

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