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C. A r c h i m a n d r i t i s l ' , J u l i a M a l a m i t s i 2 , E l i a s Belkasl , Alexandra D. Varvarigou' , K y r i a k o s C o t s y f a k i s l , Gregory Sivolapenko3, John Zorzos4, Demosthenes V . Skarlos5 and Charalambos P r o u k a k i s 2 . SUMMARY The present study r e f e r s t o t h e p r e p a r a t i o n o f a 99m-Technetium l a b e l l e d murine monoclonal a n t i b o d y f o r c l i n i c a l a p p l i c a t i o n . The monoclorla a n t i b o d y was incubated w i t h a 2 0 f o l d molar excess of 2-iminoth olane. The f r e e t h i o l groups c r e a t e d , were capable o f b i n d i n g reduced technetium. L a b e l l i n g took p l a c e through an exchange r e a c t i o n w i t h 99m-Technetium-Glucoheptonate. The l a b e l l i n g c o n d i t i o n s were s t u d i e d e x t e n s i v e l y . Diverse chromatographic methods (ITLC, HPLC) were developed i n o r d e r t o study t h e Address any correspondance t o : * D r . S . C . A r c h i m a n d r i t i s ,

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A procedure for the radiochemical purity control of colloid and particulate 99mTc-labeled radiopharmaceuticals is described. The proposed technique is based on the use of two chromatograms, using in both, 15% H3PO4 as solvent and impregnated glass fiber media (Gelman ITLC type SG) as stationary phase. A pretreatment of the radiopharmaceutical with 6 N NaOH is involved prior to one chromatographic run. The procedure is fast and the different species (free pertechnetate, 99mTc-Sn-colloid and labeled 99mTc) in a 99mTc-labeled radiopharmaceutical can be determined accurately and with reliability.

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