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The presence of B substance in saline extracts of albumen glands of Helix pomatia has been reported by Kim et al. [5] and the agglutination of Hela cells by anti-Aiiei mentioned by Uhlenbruck et al. [10]. We report in this paper serological differences between related species of snails revealed by haemolysis, agglutination of Hela cells and presence of B-like substance. The same snail substances have been used as in Part I of this report. Saline extracts of seeds were prepared as described in [2] and anti-BsT was the same as that used for experiments in Part I of this report.

The results of agglutination and agglutination-inhibition experiments with two sponge extracts (Axinella sp. and Cliona celata) and three snail extracts (Achatina granulata, Theba pisana and Helicella virgola, banded form) are reported. The extracts which show D-galactose specificity and are also inhibited by sugars related to D-galactose or containing it, have been subdivided into 1. a subgroup with no blood group specificity to which belong Axinella sp., Cliona celata and Ricinus communis extract and 2. another subgroup with B-like specificity to which belong extracts of Helicella virgata (banded form), Bandeiraea simplicifolia and Fomes fomentarius extracts. Extracts of subgroup 1. but not of subgroup 2. are inhibited by Pn XIV polysaccharide. In spite of anti-B-like specificity, extracts belonging to subgroup 2. are just as well or even better inhibited by NAcgal than by D-galactose. The extracts of Achatina granulata and Theba pisana lack D-galactose specificity. Achatina granulata extract was inhibited by NAcgal, NAcglu and NANA; Theba pisana extract only by NANA. Theba pisana extract agglutinated only neuraminidase-treated red cells, Helicella virgata (banded form) extract only enzyme-treated B cells. inhibited by NAcgal, NAcglu and NANA; Theba

Agglutination-inhibition, precipitation and precipitation-inhibition tests have been performed with 2 sponge and 4 snail extracts using as inhibitors a number of biological materials and some glycoproteins and glycolipids. Some human salivas and fluids from ovarian pseudomucinous cysts inhibited, others did not inhibit. Inhibiting power was unrelated to presence of ABH substances. Some snail and seed extracts inhibited haemagglutination by Axinella sp. extract and some snail extracts haemagglutination by Cliona celata extract. Axinella sp. extract precipitated non-haemagglutinating Achatina granulata and Hedleyella falconeri snail extracts; this precipitation was inhibited by the same sugars which also inhibit haemagglutination by Axinella sp. extract.

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