The effect of two active ingredients of fungicides on pure culture of Botrytis cinerea Pers was assessed. The analysis was carried out for 16 possible combinations of cyproconazole and fludioxanil, one of which was a control one. According to the results of measurements of the dry weight of mycelium in an accumulative culture, a diagram was constructed and two-factor analysis of variance was given. It has been established that the combined effect of these fungicides has a significant effect on the growth of mycelium. The following combinations had significant effect: fludioxonil at maximum concentration (0:10), 1 part of cyproconazole and 10 parts of fludioxonil (1:10), 5 parts of cyproconazole and parts of 10 fludioxonil (5:10), 10 parts of cyproconazole and 5 parts of fludioxonil (10:5), and the maximum concentrations of two substances (10:10).

No abstract

Fusarium infection promotes losses of yield and death of plants. Fusarium infections are dangerous because grains contain fusariotoxins that cause intoxication in humans and animals, as well as necrosis of plant tissues and changes in plant growth. Fungicides inhibit the growth of mycelium, but strobilurins are known to promote the production of mycotoxins by fungi, and triazoles, on the contrary, reduce the production of deoxynivalenol. The toxigenic effect of fungicides was assessed by biotest method. Wheat grains were germinated with the addition of disinfected culture fluid. The culture fluid after the cultivation of Fusarium oxysporum with addition of azoxystrobin and pyraclostrobin in small doses was toxic for grains. The death of a significant part of the grains was noted in it. The fungistatic effect of small doses was not expressed. Therefore, the adaptation of the fungus was realized as a change in metabolism. High doses of azoxystrobin and pyraclostrobin in culture fluid increased seed germination. Those doses had a fungistatic effect. The addition of carbendazim increased the fungistatic effect and decreased the toxicity of the strain. Development of combined fungicides with sufficient doses of active substances reduces the risk of inhibition of the growth of the protected crops.

The effect of two active substances of fungicides on wheat (cv. Julius) and barley (cv. Gloria) was assessed. The analysis was performed in three replications for 15 possible combinations of ciproconazole and fludioxanil, with two control samples in addition. As a result, parts of normal, infected, abnormal and non-germinated seeds was analyzed. The number and diameter of the fungi colonies were searched. The following combinations of cyproconazole and fludioxonil were most effective: 2:4, 4:1, 4:2 respectively.