Hydrogels based on chitosan derivatives, sodium alginate with acrylamide and acrylic acid were obtained by free radical solution copolymerization method in the presence of ammonium persulfate as initiator and urotropin and N,N methylene bisacrylamide as crosslinking agents. The formation of hydrogels was proved by extraction and IR spectroscopy. It is shown that hydrogels are able to fix the temporary form in iron (III) chloride solutions and reclaim it in solutions of ascorbic acid in less than 2 hours. Hydrogels based on sodium alginate have the best physical-mechanical characteristics compared with chitosan-based - the strength reaches 0.7 MPa and 0.11 MPa, the elasticity modulus is 1.02 MPa and 0.17 MPa at 65% deformation, correspondingly.

The graft-polymerization of N, N-dimethylaminoethylmethacrylate (DMAEMA) on chitosan (CTS) was performed in the presence of ammonium persulfate (PSA) at a temperature of 323K. The effect of the DMAEM concentration in the reaction mixture on the copolymer yield, the degree and effectiveness of grafting, as well as on the physical-mechanical properties of films obtained on the basis of synthesized copolymers was studied. The copolymers formation was prooven by gravimetric method, extraction, and IR spectroscopy. Chitosan-graft-DMAEM copolymers of various compositions were obtained. The grafing degree reached ~ 350 wt. % with the ratio [DMAEMA]/[CTS] = 5 (mol/(base-mol), the grafting effectiveness was ~ 86 wt. % with the ratio [DMAEMA]/[CTS] = 1 (mol/(base-mol). It was established that the changing the copolymers composition lead to variation of tencile strength and deformation characteristics of films widely from 1.4 MPa to 25.7 MPa and from 34% to 413%, correspondingly.

The anticancer activity of the multicomponent nanostructured drug “gold nanoparticles – apitoxin - chitosan” was revealed on white rats with implanted carcinoma under injecting encompassing of neoplasms. Drug at therapeutic doses when the apitoxin containing in it one degree lower than toxic (DL 50) effectively inhibits the growth of implanted tumor. On the 28th day after the course administration the external surface area of the tumor decreases in 5 times compared to control animals. Antitumor effect is also reflected as the normalization of free-radical processes. Indicators of biochemiluminescence (Imax) of animals reduces from 250 mV with implanted tumor in the control group to 150 mV of animals with implanted tumor which injected the drug. The value of leukocyte coefficient which characterizes the status of the animals organism (normal or stress), in experiments with the drug do not significantly differ from the normal values (5.36 ± 0.72; 6.73 ± 1.09 respectively) and appreciably higher than in control group of animals with implanted tumor that the drug is not administered (1.91 ± 0.15). Leukocyte ratio 1.91 shows that control animals are under stress.

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