Solid-phase peptide synthesis of dipeptide (histidine-β-alanine) as a chelating agent examined by common N-9-fluorenylmethyloxycarbonyl-N-trityl-L-histidine and tert-butyloxycarbonyl-β-alanine-OH amino acid derivatives. Trityl chloride resin was used as a carrier resin. The molecular structure of the dipeptide was definite by using different methods such as ultraviolet visible (UV-Vis), Fourier transform infrared (FTIR), proton ( 1 H) nuclear magnetic ressonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) and the chelating property of synthesized dipeptide was investigated for removing of metal ions Al 3+ , Cu 2+, Hg 2+ and Pb 2+ in vitro. In addition, the pharmacological and biological activities of dipeptide were examined by prediction of activity spectra for substances (PASS) program.

Chelation therapy is the administration of chelating agents to remove heavy metals from the body. Chelation therapy has a long history of use in clinical toxicology. For the most common forms of heavy metal intoxication -lead, arsenic, or mercury. A number of chelating agents are available are ethylenediaminetetraacetate (EDTA), dimercaptosuccinic acid (DMSA), 2,3-dimercapto-1-propanesulfonic acid (DMPS), alpha lipoic acid (ALA) and carnosine. They used in conventional and alternative medicine. In this work synthesize of carnosine has been studied. Carnosine (beta-alanyl-L-histidine) is a dipeptide of the amino acids beta-alanine and histidine. Carnosine is found naturally in healthy muscles, heart, brain, liver, kidneys and other tissues. N-Protected amino acids are important intermediates in organic synthesis. The use of N-phthalylamino acids as coupling agents in the synthesis of peptides has been studied by several groups of investigators. Hydrolysis of the phthalyl group with hydrazine at room temperature affords a direct synthesis of L-Carnosine. The molecular structures of the compounds were definite by using UV-Vis and FT-IR spectroscopy.