Investigation of the protective effect of boric acid against hepatotoxic and nephrotoxic injury induced by acrylamide in rats.

Deltamethrin (DM) is an insecticide and is widely used around the world. Rosmarinic acid (RA) is found in herbs and spices in the Lamiaceae (mint) family and has antioxidant, anti-inflammatory and anti-apoptotic effects. We aimed to examine the protective efficacy of Rosmarinic acid in preventing the toxic effects of Deltamethrin. A total of 28 male rats were used in this study. Group 1: Control group, Group 2: RA group, RA was given 20 mg/kg peroral (p.o.) for 7 days, Group 3: DM group, 35 mg/kg/dose of DM was given 24 hours before sacrification as a single dose by gavage, Group 4: RA+ DM group. RA was given for 7 days, at the end of the 7th day, a single dose of 35 mg/kg DM was given and the animals were sacrificed after 24 hours. BUN, creatinine, AST and ALT values of the RA+DM group were lower than the DM group. Serum TAS and TOS values were higher in the DM group compared to the RA+DM group. The damage scores of the DM group were higher than those of the RA+DM group. Rosmarinic acid has been shown to have positive effects in the treatment of deltamethrin-induced nephrotoxicity and hepatotoxicity.

Wound healing is a dynamic process that is initiated in response to injury. 1 This process takes place in 4 main steps: inflammation, proliferation, matrix deposition and remodelling. 2,3 There are many factors that have detrimental effects on wound healing, and there have been many attempts to analyse these, as well as clinical studies which aim to find new methods to improve these healing processes. 4 Wound healing depends on neoangiogenesis, activation of the local immune response and the presence of

Total globozoospermia is diagnosed by the presence of 100% round-headed spermatozoa without acrosomes. It is still unclear whether patients whose ejaculate contains both normal and globozoospermic cells (partial globozoospermia) suffer from a variation of the same syndrome. Affected men may experience decreased fertility and even infertility. In some cases, an increased number of cells with DNA fragmentation has also been observed in patients with globozoospermia..In this study, standard semen analysis methods in accordance with WHO criteria were applied to infertile male patient groups consisting of 20 normozoospermic and 20 oligozoospermic individuals who were admitted to our clinic. Age, sperm parameters (volume, vitality, concentration, total motility and morphology) were determined and statistically analyzed in normozoospermic and oligozoospermic infertile men.Sperms were stained with the Eosin-Nigrosin method and were visualized under an immersion lens light microscope and evaluated for vitality. The slides were stained using sperm staining solutions with the Spermac technique and the sperms were evaluated morphologically. Sperm DNA fragmentation damage was evaluated by acridine orange staining method.Our results revealed that sperm morphological features (Kruger test) and sperm DNA fragmentation, obtained with various staining techniques, are important in the clinical approach to male infertility and ART methods, and should be used together.

Benzene is an aromatic hydrocarbon and a colorless and odorous liquid. It is used in the production of plastics, detergents, pesticides, and other chemicals. Serious deterioration is seen in sperm production of men who are exposed to hydrocarbons such as ethylbenzene, toluene, xylene, and aromatic solvents found in paints, lacquers, adhesive-like substances in their professions. The purpose of this study was to examine the possible effects of benzene on testicular tissues via histopathological and TUNEL staining methods. In this study, healthy 18 Wistar albino male rats, with a mean age of 8-10 weeks weighing 250-300 g were used. Rats were divided into 3 groups; Group I was the control group. Group II was administered 1 ml/kg benzene for 9 days and Group III 1.5 ml/kg benzene for 5 days via orogastric tube. At the end of the experiment, all rats were decapitated, and their testicular tissues were excised. Stained with Haematoxylin and Eosin, Johnsen scoring was performed for each group. Apoptosis was demonstrated with TUNEL method, marked with the Image J program and statistical analysis was performed. Benzene exposed rat testicular tissues depicted thinning of seminiferous tubule epithelium cytoplasmic vacuoles, affusion of seminiferous tubule epithelium to the lumen, and affusion in spermatogenic cells (desquamation), degenerative changes in germ cells (spermatogonia), degenerative tubule structures, disorganized interstitial tissues, and absence of epithelial integrity were observed. Compared with the control group, the rats exposed to benzene revealed a significant increase in apoptotic cells in the seminiferous tubules via TUNEL staining. It was thought that exposure to benzene resulted in degeneration and increased apoptotic cells in the testicular tissues.