A new electrospinning process was developed for preparing TiO 2 nanofibers using a water-soluble Ti-precursor, [bis(kappa1O-hydroxo)(bis(kappa2O,O 0 -lactato)titanium(IV)] commonly known as titanium(IV) bis (ammonium lactato) dihydroxide (TiBALDH). The importance of the study is justified by the fact that Ti-precursors used for electrospinning, sol-gel, hydrothermal and other fiber synthesis processes are mostly non-water soluble. Accordingly, anatase TiO 2 nanofibers of diameter between 20 and 140 nm were synthesized by electrospinning and annealing. Polyvinylpyrrolidone (PVP) and different concentrations of TiBALDH were dissolved in a mixture of water, ethyl alcohol and acetic acid to optimize the electrospinning conditions. The thermal decomposition and fragmentation of PVP, TiBALDH and the fibers with 50% mass fraction of TiBALDH were studied by TGA-MS measurements. The fibers were then annealed at 1°C min -1 until 600°C. The TiO 2 fibers were characterized using SEM-EDX, FTIR and XRD