ResumoA dor é definida como uma desagradável experiência sensorial e emocional associada a um dano atual ou potencial do tecido, ou descrita em termos deste dano, recebendo a denominação de "5º sinal vital". Objetivou-se, neste estudo, avaliar a influência da intensidade da dor sobre as respostas nas escalas unidimensionais de mensuração de dor em idosos e adultos jovens internados no Hospital Universitário do Oeste do Paraná. Foi avaliada a dor de dois grupos de pacientes, idosos (GI) e adultos jovens (GII), contendo 100 indivíduos cada grupo, do período de abril a julho de 2006, que foram selecionados conforme critérios de inclusão e exclusão da pesquisa. A avaliação da dor foi realizada através das escalas: Escala Visual Analógica (EVA), Escala Visual Numérica (EVN) e Escala de Fáceis (EF). Os resultados apontaram forte nível de concordância entre as escalas no grupo GII, apresentando significância estatística (p<0,05) nas três classificações de dores, porém no grupo GI observou-se fraca concordância entre as escalas (p<0,05) da EVN na classificação da dor leve. Conclui-se que os resultados demonstraram a existência de influência da intensidade de dor sobre as respostas das escalas no grupo GI, porém no grupo GII não se observou influência da intensidade da dor sobre as respostas. Palavras-chave: Dor. Medição da dor. Idoso. AbstractPain is defined as an unpleasant sensorial and emotional experience associated to a current or potential damage to the tissue, or described in terms of this damage, as the "5 th vital sign". The purpose of this study was to evaluate the influence of the pain intensity on the responses in unidimensional scales of pain measurement in elderly people and young adults who were admitted to the Hospital Universitário do Oeste do Paraná. The pain was evaluated in both groups, the elderly (GI) and young adults (GII), 1 Estudo realizado no Hospital Regional do Oeste do Paraná.

Revista da Sociedade Brasileira de Medicina Tropical 44(5):636-637, set-out, 2011Occurrence of 102 cases of paracoccidioidomycosis in 18 months in the Itaipu Lake region, western Paraná

In the present work, the gene xynB2, encoding a β-xylosidase II of the Glycoside Hydrolase 39 (GH39) family, of Caulobacter crescentus was cloned and successfully overexpressed in Escherichia coli DH10B. The recombinant protein (CcXynB2) was purified using nickel-Sepharose affinity chromatography, with a recovery yield of 75.5 %. CcXynB2 appeared as a single band of 60 kDa on a sodium dodecyl sulfate polyacrylamide gel and was recognized by a specific polyclonal antiserum. The predicted CcXynB2 protein showed a high homology with GH39 β-xylosidases of the genus Xanthomonas. CcXynB2 exhibited an optimal activity at 55 °C and a pH of 6. CcXynB2 displayed stability at pH values of 4.5-7.5 for 24 h and thermotolerance up to 50 °C. The K (M) and V (Max) values were 9.3 ± 0.45 mM and 402 ± 19 μmol min(-1) for ρ-nitrophenyl-β-D-xylopyranoside, respectively. The purified recombinant enzyme efficiently produced reducing sugars from birchwood xylan and sugarcane bagasse fibers pre-treated with a purified xylanase. As few bacterial GH39 family β-xylosidases have been characterized, this work provides a good contribution to this group of enzymes.

Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb) and corresponds to prevalent systemic mycosis in Latin America. The aim of the present work was to evaluate the dose response effect of the fungal yeast phase for the standardization of an experimental model of septic arthritis. The experiments were performed with groups of 14 rats that received doses of 103, 104 or 105 P. brasiliensis (Pb18) cells. The fungi were injected in 50 µL of phosphate-buffered saline (PBS) directly into the knee joints of the animals. The following parameters were analyzed in this work: the formation of swelling in knees infused with yeast cells and the radiological and anatomopathological alterations, besides antibody titer by ELISA. After 15 days of infection, signs of inflammation were evident. At 45 days, some features of damage and necrosis were observed in the articular cartilage. The systemic dissemination of the fungus was observed in 11% of the inoculated animals, and it was concluded that the experimental model is able to mimic articular PCM in humans and that the dose of 105 yeast cells can be used as standard in this model.

ResumoUma das tarefas mais importantes do sistema do controle postural humano é o equilíbrio do corpo sobre a pequena base de apoio fornecida pelos pés. Como um sensor de gravidade, o sistema vestibular é uma das ferramentas mais importantes do sistema nervoso no controle da postura. O presente estudo teve como objetivo estudar a influencia do sistema vestibular no equilíbrio de adultos jovens, por meio da Posturografia Dinâmica Foam-laser (FLP). Para a realização do estudo, foram convidados 30 voluntários adultos jovens, acadêmicos do curso de educação física da UFSM, que foram submetidos à avaliação do controle postural através de FLP e plataforma de força simultaneamente, durante o Teste de Organização Sensorial (TOS) I e V. Os resultados revelaram que houve moderado e forte índice de correlação entre os valores obtidos nos TOS I e V e a área de deslocamento do centro de pressão (CP) registrada pela plataforma de força. Conclui-se que a FLP é um método muito útil para avaliação do equilíbrio, e seus resultados demonstraram boa correlação com a área de deslocamento do CP. Palavras-chave: Posturografia Dinâmica Foam-Laser. Equilíbrio. Sistema vestibular. AbstractOne of the most important tasks for the human postural control system is to maintain body balance on a small support base provided by the feet. As a gravity sensor, the vestibular system is one of the most important nervous system tools in posture control . The objective of this work was to study the influence of the vestibular system on the young adults' balance control through Dynamic Posturografy Foam-laser (FLS). Participated in the study 30 young adults from the UFSM's physical education undergraduate course, who volunteered to go under postural control evaluation through the FLP and platform of power, simultaneously, during the Sensorial Organization Test (TOS) I and V. Results showed that there was a