Feeding habits of Bryconamericus stramineus were described in different streams of Guiraí River Sub-basin in Mato Grosso do Sul State, Mid-West of Brazil. Fishes were sampled bi-monthly between October/06 and August/07 in seven streams and the samples were separated in two seasons: dry and wet. A total of 960 individuals were captured. B. stramineus showed an insectivore feeding habit, consuming seventeen food items. The feeding tactic of preys capture varied between the seasons and the locations. During the wet season the most important items were Hymenoptera, Diptera and Coleoptera, while Megaloptera and Diptera were the most important during dry season. In all analyzed streams, B. stramineus showed a diet basically composed by insects; the other items spent sometimes, corroborating the literature. Resumo: Os hábitos alimentares de Bryconamericus stramineus foram estudados em riachos da sub-bacia do rio Guiraí, no estado de Mato Grosso do Sul, centro-oeste do Brasil. As amostragens foram realizadas bimestralmente em sete riachos na bacia do rio Guiraí, Alto Rio Paraná, de outubro/2006 a agosto/2007. Foram capturados 960 exemplares. As amostragens foram divididas em estação chuvosa e estação seca. B. stramineus apresentou hábito alimentar insetívoro; a espécie utilizou 17 itens alimentares, sendo que a tática de consumo das presas variou entre as estações a as localidades. Durante a estação chuvosa nos estômagos prevaleceram as Ordens Hymenoptera, Diptera e Coleoptera, durante a seca predominaram as Ordens Megaloptera e Diptera. Em todos os riachos analisados, B. stramineus apresentou dieta basicamente composta por insetos, sendo os demais itens consumidos ocasionalmente, confirmando a literatura. Palavras-chave: dieta, insetivoria, caracídeo.
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