Rabbit polyribosomal globin messenger RNA (mRNA) and messenger ribonucleoprotein (mRNP) were labelled at the 3' poly(A) tail to high specific activity with T4 RNA ligase and [5'-(32)P]pCp without consequent loss of functional activity. Labelled message was translated in both micrococcal nuclease treated and untreated rabbit reticulocyte lysates, as shown by the formation of labelled polyribosomes. The utilisation of labelled messenger was abolished by T2 toxin or sodium fluoride which are known to inhibit protein synthesis.Images
Recent proposals to create new federal lands and management jurisdiction in Alaska, associated with the Alaska Native Claims Settlement Act (1971) and pending government legislation, have combined to bring this Native industry into prominence once again. Based on an interdisciplinary study undertaken from 1976 through 1977, contemporary herding is discussed, set against the background of history, the economic considerations and the unique Native culture and lifestyle. Reindeer herding has become an integral part of the social organization, value and cultural systems, as well as economy, of northwestern Alaska. Changes in the industry, whether to improve herding operations or as a result of government legislation, would greatly affect the people and economy of the region. &8SUM8. Les recentes propositions de creer de nouvelles terres fkderales ainsi qu'une juridiction de direction, en Alaska, associee 21 une reglementant les plaintes des indigenes; d'Alaska et A une legislation gouvernementale en suspens, tout cela s'est combine pour mettre de nouveau en vedette le travail des indigenes. L'autre discute de la harde contemporaine, B partir d'une etude interdisciplinaire, enterprise de 1976 B 1977; c'est un defi A histoire, aux considerations economiques, B la culture et au style de vie des indigenes. La harde de rennes est devenue une partie integrale de l'organisation sociale, des systemes de valeur et de culture, tout autant que d'economie dans l'Alaska du N.W. Les gens et l'economie de la region seraient grandement affectes par des changements dans leur labeur, destines B ameliorer les actions concernant les troupeaux ou comme resultant d'une legislation gouvernementale. Traduit par Alain de Vendegies, Aquitaine Company of Canada Ltd.
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