A continuous ultraviolet spectrophotometric assay for angiotension I converting enzyme has been developed utilizing amino-terminal-blocked tripeptides as substrates. Z-Phe(N02)-His-Leu, Z-Phe(N02)-Gly-Gly, and Hip(NC>2> Gly-Gly have been synthesized as effective substrates for angiotensin I converting enzyme. These substrates have facilitated the partial purification and characterization of angiotensin I converting enzyme from calf lung. This enzyme was
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