Nanotechnology is a fast-growing field of science. Nanoparticles get much attention due to their unique physicochemical, optical and thermal activities. Silver nanoparticles have been used in experiments to treat infectious diseases. The goal of the research was to make silver nanoparticles using Euphorbia hirta extract, physically characterize the nanoparticles obtained and, to evaluate silver nanoparticles' antibacterial properties. The leaf extract of E. hirta (asthma weed) was used for the reduction of 1 mM silver nitrate (AgNO 3 ) solution to silver nanoparticles (SNPs). SNPs were made by combining 50 mL of aqueous plant extract with 250 mL of AgNO 3 solution to make SNPs. The mixture was monitored for two hours. The synthesized SNPs were characterized by UV-Vis, FTIR spectroscopy and particle size. The antimicrobial activity of the SNPs was tested against Escherichia. coli, Pseudomonas. aeruginosa, Salmonella. typhi, Bacillus. subtilis and Candida. albicans. The reaction medium's hue shifted from yellow to brown. The results of the UV-vis analysis of the particles showed that at 430 nm the particles had the maximum absorption (λ max) within 2 hours. The FTIR identified carboxylic acid and other functional groups. The polydispersity index (PDI) and Z-average particle size were found to be 0.426 and 274 nm, respectively. The results of the antimicrobial studies showed sufficient growth inhibition of the bacteria by the SNPs the minimum inhibitory concentration (MIC) ranging from 7 µg-10 µg. It was concluded that SNPs were synthesized using E. hirta leaf extract. The synthesized SNPs possess good activity against pathogenic microorganisms.