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As for the nutritional requirements of Salmonella paratyphi A, Lederberg (1) has already given a short description in his paper on the nutrition of Salmonella. Result observed on him shows that among the total 8 strains of Salmonella parat,yphi A examined, 5 were capable of growing on a simple synthetic medium containing glucose, salts and asparagine, and 2 required tryptophan (or indol) or both methionine and triptophane for growth. The nutritional requirement of the remaining one has not been determined. Recently, Suzuki and Sakaguchi (2) reported that 3 strains of Salmonella paratyphi A grew well on a medium containing glucose, tryptophane cystine and salts. Present authors have examined the cultures of Salmonella paratyphi A, some of which had been kept in their laboratory and the others* Isolated newly in the Komagome Hospital for Infectious Diseases. The experimental results hereof are as summarized in the following. EXPERIMENTAL RESULTS (1) Growth factors required: Among the total 16 atrains of Salmonella paratyphi A examined, 15 were proved to be able to grow in the following simple synthetic culture medium (Medium A) : Glucose 0.2 gm Tryptophane 0.02 gm NaCl 0.5 gm MgSO4 0.02 gm (NH4)H2PO4 0.1 gm K2HPO4 0.1 gm Aq. das. 100 ml * These strains were forwarded to us by courtesy of Dr. K. Kobari of the Komagome Hospital for Infectious Diseases.

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This paper gives descriptions of the type-distribution of Salmonella and Shigella isolated in various parts of Japan during the year 1952 and forwarded to our laboratory for type identification. NAKAYA et al In the early period of the outbreak, several cultures were forwarded to our laboratory for type identification and determined to be classified as each one of the above three types. The results of the examinations on the most of cultures isolated and forwarded were showed in table 4. The cultures recovered from patients were

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