This study assessed the effect of oral ad ministration of aqueous extract of Telfairiaoccidentalis (TO) leaves on liver biochemical indices in Wistar albino rats. Fifty rats weighing 150-200g were div ided into 2 groups A and B, to test for acute (10 days) and sub-acute (4 weeks) effect respectively. Each group consisted of 25 rats, which were fu rther div ided into 5 sub-groups 1, 2, 3, 4 and 5. Sub-group 1 served as control and was orally gavaged with standard animal feed only, while sub-groups 2, 3, 4 and 5 were the test groups. In addition to the standard animal feed, 500mg/kg, 1000mg/ kg, 2000mg/kg and 4000mg/ kg of the extract was orally gavaged to the test groups 2, 3, 4 and 5 respectively. The standard animal feed g iven to the control and test groups contained the same amount of daily calories and protein. The acute and sub-acute administration of the extract caused significant differences in values of Aspartate amino Transferase(AST), Aspartate to Alanine amino Transferase(AST/ALT) rat io, Total protein, A lbumin, Globulin, Albu min/ Globulin ratio, Total body weight, Total and Conjugated bilirubin (P < 0.001) between test and control groups. Seru m levels of other parameters were statistically non-significant. There was no obvious evidence of hepato-toxicity detected in this study.