Gender Dysphoria is one of the most important psychiatric disorders whose complications include the problems occurring after sex reassignment surgery. Methods: This study investigates sexual function and satisfaction in patients with Gender Dysphoria, both operated and non-operated patients, from 2011 to 2013. We used a crosssectional analysis, and by referring to the Legal Medicine Center in the province of Fars, 70 patients with Gender Dysphoria (including 35-operated male to female and 35 non-operated male to female) were selected using the non-probability sampling method. The study data were collected by sexual satisfaction standard questionnaires, sexual function index, and Beck scale for suicidal ideation were used. The results were analyzed using SPSS V. 22. Results: According to the results, no significant difference was observed between the operated and non-operated groups in terms of sexual satisfaction. However, there was a significant difference in terms of sexual function among operated group compared to the non-operated group, at the significance level of 0.05. Conclusion: Based on our finding, the patients with sex reassignment surgery have found their identity, mental relaxation, and coordinated behavior with their genders. New sexual identity heals patients' relationship with society, presents individual in community with suitable social status and reasonable sexual function for achieving normal life.