Background The concept of patient safety is an essential component of health care systems and is one of the key pillars of quality in health care organizations. One of the most important factors related to the safety of patients is the spiritual health of staff and patients. Accordingly, this study strived to explain the status of patient safety culture and its relationship with spiritual health from the perspective of health care providers in teaching hospitals of Hamadan.Methods This study was a qualitative content analysis study with a conventional approach using semi-structured open-ended interviews with samples selected by purposeful sampling technique to achieve data saturation. The proposed method of Granheim and Landman (2004) was also used for the qualitative content analysis of the data.Results In this study, 5 themes and 11 sub-themes were obtained from the participants' experiences. These included: continuous and dynamic training and upgrading of safety skills, attention to spirituality and conscientiousness and work commitment, effective communication and teamwork, equipping human and logistical resources based on the principle of care, accurate recognition of instructions, and error control.Conclusions The evaluation of safety culture clarifies the perceptions of safety participants in the organization and the attitude of managers and employees towards safety issue which can lead to the development of safety culture and quality improvement.