The present work reports the isolation of eight compounds from Pestalotiopsis sp. EJC07 isolated as endophytic from Bauhinia guianensis, a tipical plant of the Amazon. The compounds (4S)-4,8-dihydroxy-1-tetralone (1), uracil (2), uridin (3), p-hydroxybenzoic acid (4), ergosterol (5), ergosterol peroxide (6), cerevisterol (7) and ducitol (8) were isolated by chromatographic procedures and identifi ed by spectral methods of 1D and 2D NMR and MS. The compound 1 is being reported for the fi rst time in the genus Pestalotiopsis.