The Canadian Entomologist 126: 103-110 (1994) This study determined the importance of Ophiostomatales fungi in the biology of individual adult Ips sexdentatus Boem. in Scots pine. We investigated factors possibly involved in success of rearing without Ophiostomatales, such as the sterilization technique, the presence of antibiotics, and the absence of yeasts. After sterilization of pupae, 866 adult beetles were reared in the media; the proportion of Ophiostoma-free mature adults was 99.2% and total mortality was about 13%. The most preferred rearing method used sterilized pupae which moulted on moistened filter paper and were transferred individually to separate vials where maturation took place on a sterilized phloem medium.The weight and size of Ophiostoma-free mature adults from semi-artificial media and, after establishment in logs, their fecundity and length of their egg galleries, were compared with those of naturally contaminated mature beetles obtained from logs. With the exception of a higher weight observed for adults matured in semi-artificial media, no essential difference was observed between the two groups. Ophiostomatales do not appear to play an essential role in the maturation and fecundity of I. sexdentatus adults. Colineau, B., et F. Lieutier. 1994. Production d'lps sexdentatus Boem. (Coleoptera: Scolytidae) adultes indemnes d'Ophiostoma et cornparaison avec des adultes naturellement contarninks. The Canadian Entomologist 126: 103-1 10. ResumeLe but de cette Ctude est de dCterminer si les champignons Ophiostomatales jouent, au niveau des individus, un r61e important dans la biologie des adultes d'lps sexdentatus sur le Pin sylvestre. Le r61e de divers facteurs dans le succbs de 1'6levage en absence d'Ophiostomatales a CtC testC, tels la technique de stkrilisation, la prCsence d'antibiotiques et la prCsence de levures. La mCthode d'Clevage la mieux adaptke a Ct C de placer les nymphes stCrilisCes sur papier filtre humide pour la mue imaginale puis, immkdiatement aprbs celle-ci, de transfCrer individuellement les jeunes adultes dans des pilluliers contenant un milieu axCnique B base de phlobme, pour leur maturation. Aprbs stkrilisation des nymphes, 866 adultes ont Ct C ClevCs dans les milieux semi-artificiels. La proportion d'adultes mQrs non contamines par Ophiostoma spp. a atteint 99.2% et la mortalit6 totale a Ct C de 13 %. Le poids et la taille des adultes murs indemnes d'Ophiostoma, ainsi que leur fCcon-ditC et la longueur de leur galerie de ponte aprbs installation sur rondins, ont Ct C comparCs B ceux d'adultes contaminks obtenus B partir de rondins. A l'exception d'un poids plus ClevC pour les adultes ClevCs sur milieu semi-artificiel, aucune diffkrence n'a Ct C observCe entre les deux groupes. Les Ophiostomatales ne semblent donc pas jouer un r61e essentiel dans la maturation et la fCconditC des adultes d'lps sexdentatus.
Root induction on in vitro adventitious Pinus monticola Dougl. shoots from mature embryos was improved after 8 weeks co-cultivation with Agrobacterium rhizogenes Conn. strain A4 or the pRi transconjugant strain R1000 as compared to controls, and to naphthaleneacetic acid and indoleacetic acid treatments. An improvement in the number and quality of roots induced was observed on co-cultivated shoots as well as co-cultivated Pinus banksiana Lamb and Larix laricina (DuRoi) K. Koch de-rooted seedlings. Changes in substrate nutrient solution did not influence rooting as much as the presence of Agrobacterium in the rhizosphere. Rooting of cuttings from 3-year-old P. monticola trees was improved in two different seedlots with A. rhizogenes A4 strain.
The Canadian Entomologist 130: 27 -54 (1998) The Agabus semivittatus-group is revised. The group, which is restricted to temperate Northtatus new species in Texas. Agabus stagninus and A. flavovittatus hybridize in southeastern Pennsylvania. The placement o f Agabus texanus Sharp as a junior synonym o f A. semivittatus is confirmed. A lectotype is designated for Agabus spilotus LeConte (=A. semivittatus). A neotype is designated for A. stagninus. A key to species is presented and, for each species, the following information is provided: description; illustrations o f taxonomically important characters; morphological measurements on selected population samples; notes on relationships, variation, and ecology; and a map o f collection localities. A cladistic analysis o f the relationships o f the group within Agabus indicated that the americanus-, spinipes-, and confusus-groups belong to the same clade as the semivittatus-group and that the seriatus-group is sister to this clade. Within the semivittatus-group, there are three well-defined subgroups as follows: A. semivittatus; A. johannis and A. astrictovittatus; and A. stagninus, A. flavovittatus, and A. texovittatus. Larson, D.J., et R.W. Wolfe. 1998. Rivision du genre nord-amiricain Agabus (Coleoptera : Dytiscidae) : le groupe semivittatus. The Canadian Entomologist 130 : 27-54. Le groupe Agabus semivittatus a fait l'objet d'une revision. Ce groupe, restreint a la zone temptree nord-amkricaine, compte six esptces: A. semivittatus LeConte, presque transcontinental, A. johannis Fall, dans la peninsule de Floride, A. astricovittatus, nouvelle espece, repartie le long de la c6te atlantique du New York au nord de la Floride et a l'Alabama, A. stagninus (Say), aussi sur la c6te atlantique du New York la Floride et le long de la c6te du Golfe jusqu'a la Louisiane, A. flavovittatus, nouvelle espece, largement repandue a l'est des Grandes Plaines, du sud de I'Ontario au Mississippi et a la partie est du Texas, mais pas sur la c6te atlantique, enfin A. texovittatus, nouvelle espkce, du Texas. I1 y a hybridation entre Agabus stagninus et A. flavovittatus dans le sud-est de la Pennsylvanie. Nous confirmons ici qu'Agabus texanus Sharp est bien un synonyme recent d'A. semivittatus. Des lectotypes ont kt6 designes pour A. spilotus LeConte (=A. semivittatus). Un nkotype d'A. stagninus a Bt B choisi. On trouvera ici une clk des espkces et, pour chaque espece, les informations suivantes: description, illustration des caracteres diagnostiques, mesures morphologiques d'kchantillons choisis de la population, notes sur les liens de parent6, sur la variation et sur l'kcologie, enfin une carte des sites de rkcolte. L'analyse cladistique de la position du groupe au sein du genre 28 THE CANADUN ENTOMOLOGIST JanuaryFebruary 1998 Agabus a rCvtle que les groupes americanus, spinipes et confusus appartiennent au meme clade que le groupe semivittatus, et que le groupe seriatus est le groupe soeur de ce clade. Au sein du groupe semivittatus, il existe trois sous-groupes bien dkfinis : (1) A. se...
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