No abstract

No abstract

Traditionally Tourism Destination Management organized and promoted a region in becoming successful tourism destination based on a wide range of knowledge and experience, both in the international and local level. The concept of Tourism Sustainable Development however has added several novel factors in this process, including sustainability performance indicators. In this study, the authors present the island of Poros in Greece where carrying capacity estimation has been applied in order to support tourism promotion in an effective and sustainable manner.

The peripheral blood is a major source of hematopoietic stem cells. Almost for two decades the peripheral blood has been mobilized, in order to enhance the CD34 + concentration. The isolated stem cells from the mobilized peripheral blood are used as an alternative, or in addition to bone marrow derived stem cells. In this paper, a new perspective is being discussed; the use of non-mobilized peripheral blood as an alternative source for hematopoietic progenitor cells. The number of isolated hematopoietic stem cells is evaluated using flow cytometry. The viability can be evaluated using the trypan blue exclusion test, the flow cytometry or automated assays. The isolated hematopoietic stem cells could be used for ex vivo expansion either in static systems or in proper bioreactor systems, prior to cryopreservation and/or transplantation.