Basal stem rot disease (BSR) caused by G. boninense affects most oil palm plants in Southeast Asia. This disease can be fatal to palm oil production. BSR shows no signs on the tree in the early stages of infection. Therefore, it is essential to find an approach that can detect BSR disease in oil palm, especially at any level of disease severity in the field. This study aims to identify biomarkers of BSR disease in oil palm stem tissue based on various disease severity indices in the field using 1H NMR-based metabolomics analysis. The crude extract of oil palm stem tissue with four disease severity indices was analyzed by 1H NMR metabolomics. Approximately 90 metabolites from oil palm stem tissue were identified.Twenty of these were identified as metabolites that significantly differentiated the four disease severity indices. These metabolites include the organic acid group, the carbohydrate group, the organoheterocyclic compound group, and the benzoid group. In addition, different tentative biomarkers for different disease severity indices were also identified. These tentative biomarkers consist of groups of organic acids, carbohydrates, organoheterocyclic compounds, nitrogenous organic compounds, and benzene. There are five pathways in oil palm that are potentially affected by BSR disease.
Indonesia has one of the largest archipelagic systems in the world, potentially affecting the complex population structures of aquatic organisms. The Scalloped Spiny Ocean (Panulirus homarus, Linnaeus 1758) has been identified widely distributed worldwide. These facts have led to questions regarding spiny lobster harvest and culture management by considering population differentiation and habitat fragmentation on complex and distinct archipelagic islands. Our investigation was conducted using high-density SNPs datasets from several spiny lobsters harvested from five locations in Indonesia. We found strong differentiation among spiny lobster populations clustered into 3 sub-populations. Environment association analysis and Fst analysis revealed outlier loci significantly associated with Sea Surface Temperature variation and potentially correlated with Sea Current-related parameters. These finding were suggested to be implemented as the essential consideration in spiny lobster management.
Basal stem rot disease (BSR) caused by G. boninense affects most oil palm plants in Southeast Asia. This disease can be fatal to palm oil production. BSR shows no signs on the tree in the early stages of infection. Therefore, it is essential to find an approach that can detect BSR disease in oil palm, especially at any level of disease severity in the field. This study aims to identify biomarkers of BSR disease in oil palm stem tissue based on various disease severity indices in the field using 1H NMR-based metabolomics analysis. The crude extract of oil palm stem tissue with four disease severity indices was analyzed by 1H NMR metabolomics. Approximately 90 metabolites from oil palm stem tissue were identified, and 20 of these were identified as metabolites that significantly differentiated the four disease severity indices. These metabolites include the organic acid group, the carbohydrate group, the organoheterocyclic compound group, and the benzoid group. In addition, different biomarkers for different disease severity indices were also identified. These biomarkers consist of groups of organic acids, carbohydrates, organoheterocyclic compounds, nitrogenous organic compounds, and benzene. There are five pathways in oil palm that are potentially affected by BSR disease.
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