Background and Objectives: The work-family conflict has many negative consequences, such as absenteeism, employee turnover, and reduced performance. This study aimed to investigate and compare perceived work-family conflict among clinical and non-clinical employees working in hospitals affiliated to Shahid Sadoughi University of Medical Sciences. Methods: This descriptive analytical cross-sectional study, was conducted on 210 employees working in Shahid Sadoughi educational hospital in Yazd city, 2016. Data collection was performed using Carlson work-family conflict questionnaire. Data analysis was carried out by t-test, Spearman correlation coefficient, and one-way analysis of variance. Results: The mean perceived work-family conflict among the study population in terms of three dimensions of time, pressure, and behavior, were 18.11+4.23, 7.91+5.09, and 18.16+4.71, respectively, which showed a significant difference between clinical and nonclinical employees (p<0.001). Furthermore, perceived work-family conflict had negative correlation with age and work experience of the personnel (r=-0.222, r=-0.237). Conclusion: Given that female employees are more exposed to workfamily conflicts compared to male ones, therefore, measures should be taken to decrease work-family conflict level through greater focus on these groups, creating an appropriate supportive environment, and avoiding rigid and inflexible rules.