Infertility is a worldwide problem affecting couples that have unprotected intercourse, so the current study investigated the possible ameliorative effect of intra testicular stem cell injection in rats subjected to gentamicin induced testicular degeneration. For this study 100 male rats were randomly allocated into 4 groups, namely, the control group (group I), gentamicin group (group II), stem cell group (group III) and gentamicin and stem cell group (group IV). Gentamicin was administered daily for 2 weeks (80 mg/kg) to groups (II and IV). Stem cells were injected intra testicular into rete testis once by the end of gentamicin administration in group (IV) or after I/P injection of saline in group III. Blood and testicular samples were collected by the end of weeks 2, 6 and 10 from group (I) and (II), and by the end of weeks 6 and 10 from group (III and IV). The obtained results showed a significant decreases in serum testosterone, testicular testosterone and estradiol, sperm counts, sperm viability and an increase in % of fragmented DNA (indicating degenerative and necrotic changes) in testicular tissue of group (II) compared to group I , III and IV. Mesenchymal stem cells administration significantly improved all altered testicular parameters, where values of group IV (except testicular estradiol) were not significantly different from those of control group (I). Conclusively, mesenchymal stem cells proved to be beneficial in rats subjected to gentamicin induced testicular dysfunctions.