Oocyte and embryo developmental competence are shaped by multiple extrinsic and intrinsic factors. One of the most extensive research areas in the last decade is the regulation of lipid metabolism in oocytes and embryos of different species. We hypothesized that differences in developmental competence of oocytes and embryos between prepubertal and cyclic gilts may arise due to distinct fatty acid profiles in follicular fluid. We found that supplementation of oocyte maturation media with follicular fluid from prepubertal pigs affected quality and development of embryos from prepubertal pigs while embryos of cyclic pigs were not affected. PLIN2, SCD and ACACA transcripts involved in lipid metabolism were upregulated in embryos originating from oocytes of prepubertal pigs matured with autologous follicular fluid. The surface occupied by lipid droplets tend to increase in oocytes matured with follicular fluid from prepubertal pigs regardless oocyte origin. The change into follicular fluid of cyclic pigs increased the efficiency of embryo culture and improved quality, while gene expression was similar to embryos obtained from cyclic gilts. We assume that the follicular fluids of prepubertal and cyclic pigs influenced the quality of oocytes and embryos obtained from prepubertal pigs which are more susceptible to suboptimal in vitro culture conditions.

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The dynamically changing world poses new challenges to urbanized areas, e.g., related to water management in the architectural and urban context. Improving retention and strengthening blue-green infrastructure can be based on technical, semi-natural and natural methods, which are less invasive. Various forms are used in the city space: flow control, detention, retention, filtration, infiltration, and treatment. The implementation of the green order strategy and shaping sustainable development in the context of designing the city’s resilience is associated with shaping spatial policy and urban planning assumptions for the transformation of public spaces and new investments in urbanized areas in crisis. The shaping of waterfronts in the city and green and blue infrastructure significantly shape the parameters of the environment and the regenerative capacity of the urban ecosystem. The aim of this work was to show the relationship between the problem of embankments and the possibilities of developing space in their proximity, including areas exposed to the risk of flooding. The relationships determined by the goal were verified in comparative studies, a repeatable method of collecting, processing, analyzing, and interpreting the obtained data was used. The issue was presented in a broader context of flood risk and water management in the area of Lake Zegrze, collisions and spatial conflicts were analyzed. The results are presented in the context of detailed water management data for the complex of Riva Zegrze facilities, which is a model example regarding the sustainable development of areas on embankments, considering their specific floodplain development possibilities. The effects of the research allowed for the formulation of conclusions, including in terms of implementation, in the field of urban and architectural design for areas associated with an embankment.

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