INTRODUCTIONGroup A rotavirus (RVA) is one of the main causative agents of diarrhea in a variety of animal species, as reported in several countries (Estes & Kapikian 2007). RVA infection is common in swine farms, and it results in decreased growth performance, increased mortality and economic loss (Papp et al. 2013).RVA belongs to Family Reoviridae, Subfamily Sedoreovirinae, Genus Rotavirus, and its genome consists of 11 double-stranded RNA (dsRNA) segments, coding for 6 structural (VP1, VP2, VP3, VP4, VP6, VP7) and 6 non-structural proteins (NSP1 through 6) (King et al. 2012).Diagnosis of RVA infection plays a central role in establishing measures for control and prevention. The methods currently available, the polyacrylamide gel electrophoresis (PAGE) technique (Herring et al. 1982) allows the detection of all rotavirus strains regardless of the group to which The results also demonstrated high intra-and inter-assay reproducibility (coefficient of variation ≤1.42%); thus, this method proved to be a fast and sensitive approach for the diagnosis of RVA in pigs.
Às principais pessoas que contribuíram com minha vitória. À minha mãe Maria Aparecida Mota Marconi que com carinho, amor, dedicação, paciência e muita perseverança mostrou-me o caminho a seguir. Ao meu pai José Vicente Marconi que com carinho, amor, paciência e segurança contribuiu para eu fazer o meu melhor. Às minhas irmãs Margareth Henye Mota Marconi e Juliane Mota Marconi que com muito amor, dedicação, paciência foram meu apoio nessa jornada. À minha afilhada Ana Júlia Alves Marconi e minha sobrinha Ana Luísa Alves Marconi, por trazerem alegria e amor aos meus dias mesmo com a distância. Ao Carlos Roberto Prudêncio pelo seu amor, paciência, companheirismo, apoio e compreensão nos momentos difíceis para a realização deste estudo.
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